Blue Ridge Saga.pdf
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We observed that the proliferation of germ cells was significantly reduced in the genital ridge of Wt1R394W/R394W embryos, compared with control embryos after sex determination at E13.5. To confirm this finding, we performed the experiment of BrdU incorporation assays in the GR of control and mutant embryos at E11.5 and E12.5, and wild-type and Wt1R394W/R394W embryos at E13.5. We found that the BrdU positive cells were reduced in the GR of the Wt1R394W/R394W embryos, compared with those of the control embryos after sex determination at E13.5 (Figure 5, A-C). The proliferation of germ cells was significantly decreased in the GR of the Wt1R394W/R394W embryos, compared with those of the control embryos after sex determination at E13.5. We confirmed that the expression level of CDKI increased in the GR of the Wt1R394W/R394W embryos, compared with those of the control embryos after sex determination at E13.5 (Figure 5, D, E). The mRNA level of CDKI (p15INK4b, p16Ink4A, P21WAF1/Cip1 and p27Kip1) that inhibited the G1/S transition was estimated by real-time RT-PCR at E12.5 and E13.5 and normalized against PGC-specific marker Stella (N, O). The mRNA level of p15INK4b was significantly increased in the GR of Wt1R394W/R394W embryos, compared with that of control embryos after sex determination at E13.5 (P, Q).
Ruby Ridge was the first major incident of the name-change controversy. In September 1994, Randy Weaver sold his cabin, approximately 2,000 m (2.8 mi) from the site of the shootout, for $22,000 to William and Colleen Anderson.[16]
The battle was the subject of author Dee Brown’s 1966 book The Moon’s Crater. Two years later, the Navy produced a film, The Battle at Ruby Ridge, based on the book, and the press began calling it the Ruby Ridge Massacre. The controversy was covered in articles, books, and newsreels. A 1967 report commissioned by the U.S. Congress, commissioned by the Subcommittee on Crime of the Committee on the Judiciary, was released in 1970. It reported that the FBI had killed four women, including one with three small children, and wounded three others, as well as wounding several dogs; one child was blinded by the FBI’s use of tear gas.[14] The Army had killed one of the four children, and the remaining three children were left with lifelong disabilities, and the family home was destroyed by fire set by an FBI sniper.[15]
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